Photosynthesis by Protoplasm Extruded from Chara and Nitella

(a) Photosynthesis with protoplasm isolated from Chara or Nitella as measured by C(14) fixation has been obtained at a rate 12 to 15 per cent of that of the whole cells. (b) Photosynthesis by cut cells of Chara or Nitella with the vacuolar sap removed was at a rate comparable to that of the whole cells. (c) Both the protoplasm and the cut cells reduced CO(2) in the light to sucrose and hexose phosphates. Other products formed were also detected by paper chromatography. In contrast, dark controls fixed the C(14) into products associated with plant respiration. (d) An important difference in the products from the extruded protoplasm was the absence of C(14)-labelled pentoses or sedoheptulose which were formed, however, by the whole or cut cells. This suggests that the most sensitive site affected by disruption of the cells may be the steps involved in the regeneration of the "C-2 acceptor" for CO(2) fixation in photosynthesis.